hplc troubleshooting check valves

  • Chromatography HPLC and Lab Supply Fluidics Fittings

    hplc valves chromatography valves. Toggle navigation. SHOPPING CART (0) Solenoid Actuated Gradient and Flow Selection Valves. Upchurch Scientific® / Rheodyne® Valves. Medium Pressure Injection Valves. Medium Pressure 6 Way Selection Valves. Medium Pressure 4 Port Switching Valves.

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  • Agilent Maintaining Your Agilent 1100 Series HPLC System

    The stainless steel (SS) 7725i and PEEK 9725i valves are the latest development and most popular injection valve for analyt ical HPLC. They have a 20 µl loop installed

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  • HPLC In Line Check Valves ASI

    In line Check Valves. ASI In line check valves are designed to limit flow to only one direction similar to diodes in an electric circuit. Valves are spring loaded to permit reliable operation in either horizontal or vertical orientation. Maximum pressure limits are 15 000 PSI and 30 000 PSI for the Ultra High Pressure.

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  • HPLC Troubleshooting 5. Problems with the Injector

    5. Problems with the Injector. These problems are usually detected while using the injection valve. Leaky injection valves are discussed in Section 3 (Leaks) A. Manual Injector Hard to Turn

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  • Check valve

    A check valve clack valve non return valve reflux valve retention valve or one way valve is a valve that normally allows fluid (liquid or gas) to flow through it in only one direction. Check valves are two port valves meaning they have two openings in the body

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  • VWR HPLC Troubleshooting.ppt

    Baseline Troubleshooting Spikes Bubbles Degas solvent Poor electrical connection loose wiring Clean and tighten detector leads check wiring Lamp relay trying to fire a dead lamp Replace lamp Electrical noise Remove source. Common sources Switching valves compressors muffle furnaces

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  • PerformancePLUS Check Valve Housing for Waters HPLC

    Learn more about PerformancePLUS Check Valve Housing for Waters HPLC Systems Restek. We enable science by offering product choice services process excellence and our people make it happen.

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  • What to Check When Solvent Delivery Pressure is Abnormally

    Potential Problems That Can Occur When Solvent Delivery Pressure Becomes Abnormally High First the following summarizes some of the problems that can occur if the pressure increases abnormally high. A. If the column packing material is exposed to too much pressure it can break apart or create flat spots being pushed further into the column.

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  • Maintenance Trouble Shooting Tips HPLC Parts by ASI

    The following tips are derived from our many years of working with HPLC pumps and are presented here to assist the chromatographer or service technician to prevent problems from occurring and diagnosing and solving them when they do occur. Check Valves! Expected Service Life The life of a check valve depends entirely upon service conditions.

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  • A Novel Performance Test for Outlet Check Valve Function

    Sep 28 2010· Check valves are a vital mechanical component of HPLC pump hardware. A detailed description of check valve function and hardware designs is given in Ref. 1. Briefly a pair of check valves (an inlet and an outlet) prevents flow from a high pressure area into the low pressure area inside of the HPLC pump head.

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  • 2008 6. Check Valves and Acetonitrile LC Resources

    Jun 01 2008· About. LC Resources training courses provide comprehensive training in HPLC LC MS bioseparations and method development. Courses cover troubleshooting systems system care and maintenance theory of separation mechanisms basic separation principles advanced method development and the latest cutting edge techniques.

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  • Check Valves Chromatography Forum

    Oct 27 2008· I very rarely have problems with check valves. I run ceramic units exclusively and rebuild my pumps check valves included annually whether they need it or not. It might seem to be overkill but my unscheduled downtime is very close to non existent and I like it that way.

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  • HPLC Troubleshooting Guide Sigma Aldrich

    Isolating HPLC Problems. In an HPLC system problems can arise from many sources. First define the problem then isolate the source. Use Table 1 to determine which component(s) may be causing the trouble. A process of elimination will usually enable you to

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  • Troubleshooting LC basics Troubleshooting LC basics

    Troubleshooting LC basics Harold McNair ia Tech USA Abstract Troubleshooting HPLC Instrumentation and separations requires a fundamental understanding of how the instrument functions and how the separation works. This chapter provides a practical guide to common HPLC problems along with more in depth information to help the reader understand the relationships between the

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  • HPLC Troubleshooting

    HPLC Troubleshooting We can help you. Click on the respective observation to display the possible causes and their prevention / remedies. General instructions for column maintenance and individual rinsing procedures can be found in the accompanying column leaflet or on the corresponding website.

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  • Check Valve Failure Chromatography Forum

    Sep 22 2005· In my experience it's almost always the outlet check valve but DR's suggestion is a good way to check both. Right now I'm having problems with an old Waters 600 pump but only have some used check valves to swap out and none of them will hold pressure. It's a choice of ordering new check valves or getting out the big hammer!

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  • Filtration Preventative Maintenance for HPLC

    Check valves control the solvent flow direction through the pump head and ensure steady pressures when sealed properly. Particulate in check valves can leak or stick causing flow and/or pressure problems. Check valve leakage is prevented by filtering HPLC grade solvents using a solvent line sinker frit flushing

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  • What to Check When Solvent Delivery Pressure is Abnormally

    Potential Problems That Can Occur When Solvent Delivery Pressure Becomes Abnormally High First the following summarizes some of the problems that can occur if the pressure increases abnormally high. A. If the column packing material is exposed to too much pressure it can break apart or create flat spots being pushed further into the column.

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  • Instrument Accessories Parts / HPLC Accessories

    Over time the piston seal in every HPLC system becomes worn and must be replaced. Our piston seals are constructed of PTFE material with an additive that reduces their tendency to cold flow and improves their wear characteristics. PTFE seals are more resistant to any HPLC solvent than UHMWPE seals but they are less wear resistant and rugged.

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  • HPLC

    TROUBLESHOOTING. n HPLC/UHPLC Columns (capillary to preparative) n SEC Columns Aqueous (GFC) and non Aqueous (GPC) n Amino Acid Analysis Faulty check valve(s) 7. Replace check valve(s) 8. Major leak 8. Tighten or replace fittings A. No pressure reading no flow B. No pressure reading flow is normal

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  • Troubleshooting Common System Problems Using Waters

    A third common system problem is a bad check valve. Check valves help to regulate flow and pressure in an HPLC/UPLC system. Over time these valves may stick and become clogged depending on the types of mobile phase used. When they begin to fail there can be noticeable chromatographic and pressure issues in a system.

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  • HPLC Trouble Shotting SlideShare

    Nov 24 2011· HPLC Trouble Shotting 1. Other problems detected by the senses of smell sight and soundWhen you have corrected the problem record theincident in the system record book to help with futureproblems. Replace pistonAir trapped in pump head Purging Degas solventsInsufficient mobile phase Replenish reservoirFaulty check valve(s) Replace

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  • A Novel Performance Test for Outlet Check Valve Function

    Sep 28 2010· Check valves are a vital mechanical component of HPLC pump hardware. A detailed description of check valve function and hardware designs is given in Ref. 1. Briefly a pair of check valves (an inlet and an outlet) prevents flow from a high pressure area into the low pressure area inside of the HPLC pump head.

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  • HPLC Troubleshooting Proteins and Proteomics

    HPLC Troubleshooting 1. INTRODUCTION Locating and Correcting the Problem Prevention 2. ABNORMAL PRESSURE record the incident in the HPLC system record book to help with future problems. Prevention Many liquid chromatography (LC) problems can be prevented with routine maintenance. Faulty check valve(s) 7. Replace check valve(s) 8. Major

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  • Check valve

    A check valve clack valve non return valve reflux valve retention valve or one way valve is a valve that normally allows fluid (liquid or gas) to flow through it in only one direction. Check valves are two port valves meaning they have two openings in the body

    Live Chat
  • Ultra High Pressure Check Valves and Cartridges

    Our UHPCV Cartridge has a superior design which maximizes lifetime while minimizing check valve leakage/pressure drop. They are manufactured with our proprietary stronger alloy which enhances absorption of structural stress thereby extending the life of the check valve. Check valves for high pressure pumps come as complete assemblies. We also custom assemble balls and seats into

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  • HPLC troubleshooting Hints and tips Chromatography

    » Chromatography » Hints and tips » HPLC troubleshooting. Chromatography / Hints and tips / HPLC troubleshooting HPLC troubleshooting Abnormal pressure No pressure reading no flow. Possible cause Solution; Faulty check valves Replace check valves Major leak Tighten or replace fittings Now pressure reading flow is normal. Possible

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  • KNAUER

    HPLC Troubleshooting guide. The ultimate guide for HPLC troubleshooting identification diagnostic problem solving and preventing

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  • Troubleshooting and Diagnostics Tips and Tricks

    System Pressure problems Low pressure Check if something has changed (column mobile phase temperature method) If nothing has changed check for leaks. No pressure ©2013 Waters Corporation 10 Air in system o Prime the pump (methanol or IPA to remove air) Air in solvent lines.Not enough solvent in bottle o Replace the solvent bottle Problem with check valves

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  • HPLC Troubleshooting Guide Sigma Aldrich

    Isolating HPLC Problems. In an HPLC system problems can arise from many sources. First define the problem then isolate the source. Use Table 1 to determine which component(s) may be causing the trouble. A process of elimination will usually enable you to

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  • Why Agilent HPLC pump not building pressure??

    I'm working with Agilent 1220 LC system. The openlab software shows that the pump is idle but its shows the pressure is zero. It looks like pump is not building pressure.

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  • HPLC troubleshooting Guide 50megs

    HPLC troubleshooting for column back pressure column leaks changing baseline tailing peaks and pump and detector problems.

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  • Used HPLC Equipment Replacing Check Valves

    Check valves are one way valves sited on the inlet and outlet of the piston chamber of an HPLC pump. As the piston moves back to draw solvent into the pump the inlet valve opens to allow solvent in from the reservoir and the outlet valve closes to prevent high pressure solvent from the system being forced back into the pump chamber.

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  • Chromatography HPLC and Lab Supply Fluidics Fittings

    hplc valves chromatography valves. Toggle navigation. SHOPPING CART (0) Solenoid Actuated Gradient and Flow Selection Valves. Upchurch Scientific® / Rheodyne® Valves. Medium Pressure Injection Valves. Medium Pressure 6 Way Selection Valves. Medium Pressure 4 Port Switching Valves.

    Live Chat
  • Care Maintenance and Troubleshooting ofTroubleshooting

    problems in HPLC. 2. Explore the reasons for these column problems. 3. Propose preventative maintenance and method Check pressure with/without column many pppressure problems are due to blockages Pump Check ValvesPump Check Valves New style 7 Cartridge G1312 60010 5 5062 8568 Old style 5062 8562 Outlet Ball Valve

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  • HPLC Introduction This troubleshooting guide contains examples of some of the most common problems observed in reversed phase HPLC (RP HPLC) separations. Four major problem areas are covered peak shape retention time changes ghost peaks and problems related to column backpressure. In addition a section on column care is

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  • Troubleshooting HPLC Injectors (Manual and Automated)

    Jun 22 2013· Troubleshooting HPLC Injectors (Manual and Automated) Sample injectors are a critical component of a chromatography system. Understanding how they operate as well as the proper techniques to use and maintain them are fundamental skills needed to operated an HPLC system.

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  • Liquid Chromatography LC Consumables

    228 52964 95 Inlet Check Valve x 228 52711 93 PE Plunger Seal (includes backup ring) x 228 52069 94 Sapphire Plunger with Diaphragm x 228 55272 41 Diaphragm (2/pack) x 228 53334 96 Outlet Check Valve x 228 35871 99 Line Filter x 228 53184 93 Outlet Tubing 0.3 x 700mm Drain Valve Assembly 228 51229 91 Drain Valve Assembly HPLC Pumps

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  • HPLC Tips and Troubleshooting 9 The Check Valve

    Jun 22 2016· This video shows the maintenance of a HPLC check valve.

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  • 3 Common Problems in Check Valve Selection DFT® Inc

    Jul 10 2014· Oftentimes the problems with a check valve are not related to the valve itself but application and other factors. So when youre problem solving or replacing a check valve keep these three common problems in mind Water Hammer. Water hammer is one of the most common check valve problems.

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  • Ultra High Pressure Check Valves and Cartridges

    Our UHPCV Cartridge has a superior design which maximizes lifetime while minimizing check valve leakage/pressure drop. They are manufactured with our proprietary stronger alloy which enhances absorption of structural stress thereby extending the life of the check valve. Check valves for high pressure pumps come as complete assemblies. We also custom assemble balls and seats into

    Live Chat
  • What causes recurring pressure fluctuations in an hplc and

    What causes recurring pressure fluctuations in an hplc and how do you stop them? We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the

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  • HPLC Tips and Troubleshooting 9 The Check Valve

    Jun 22 2016· This video shows the maintenance of a HPLC check valve.

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  • HPLC Bombas

    How to avoid pump problems using critical buffers and low pressure gradient systems. Introduction. The check valves constitute the heart of an HPLC pump. Valves that do not function properly cause poor or missing chromatographic results. The entire HPLC system depends on the function of the pump valves.

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  • Inlet and Outlet Valves Agilent

    Agilent produce a variety of inlet and outlet valves designed to ensure the ongoing stability and performance of pump systems in LC applications. Manufactured to Agilent's high levels of quality security and integrity; LC inlet and outlet valves can be provided for a range of LC pumps.

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  • KNAUER

    HPLC Troubleshooting guide. The ultimate guide for HPLC troubleshooting identification diagnostic problem solving and preventing

    Live Chat
  • HPLC Troubleshooting Guide

    Isolating HPLC Problems In an HPLC system problems can arise from many sources. First define the problem then isolate the source. Use Table 1 (page 5) to determine which component(s) may be causing the trouble. A process of elimination will usually enable you to pinpoint the specific cause and correct the problem. How to Prevent Mobile Phase

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  • Valco Analytical HPLC Injectors and Switching Valves

    Valves for analytical high performance liquid chromatography can be actuated manually or automated with pneumatic or electric actuators. A complete valve product number includes information about the valve actuator and mounting hardware. Valves include stainless steel nuts and ferrules. Order sample loops separately.

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  • Troubleshooting HPLC Pump Pressure and Retention Time SCIEX

    Close the purge valve. Perform flow rate check. Leak check Remove the LC tubing from the HPLC column and install an inline connector with a stainless steel plug on the end to completely seal the LC line. On the front panel of the pumps press the VP button.

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